AccuNext 单细胞全基因组扩增试剂盒

产品货号:AG12553 / AG12554

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价格范围:¥2,000 至 ¥7,000

产品描述
本产品是专门针对 1~10 Cells 或 15 pg~100 pg 基因组 DNA 等微量样本研发的无差别全基因组扩增 (Whole Genome Amplification, WGA) 试剂盒。经过优化的酶和 Buffer 体系,可在 3 h 内完成细胞裂解、预扩增、扩增等步骤,获得微克级别的扩增产物。本产品操作简单,扩增获得的产物均一性好、重复性高,可广泛适用于全基因组测序、外显子测序、拷贝数变异(CNV)分析、单核苷酸变异(SNV)分析、qPCR 分析、基因芯片分析、胚胎植入前遗传筛查(PGS)等应用。
产品优势
1. 超高灵敏度:能够以单细胞或者微量 DNA 为模板实现均匀地全基因组扩增。
2. 精心优化的反应体系,单管操作简单,可在 3 h 内获得均一性好、重复性高的微克级扩增产物。
3. 单管操作,整个过程不需要纯化与转管,减少过多的操作带来的样本损失与污染,确保实验数据的准确性。
4. 扩增均一性好、灵敏度高等特点,可广泛适用于全基因组和外显子测序、拷贝数变异(CNV)分析、单核苷酸变异 (SNV)分析、qPCR 分析、基因芯片分析、胚胎植入前遗传筛查(PGS)等应用。
产品组成
组分 AG12553 AG12554
Cell Lysis Buffer 60 μl 240 μl
Cell Lysis Enzyme 5 μl 15 μl
AccuNext  Pre-Amp Buffer 96 μl 384 μl
AccuNext  Pre-Amp Enzyme 24 μl 96 μl
AccuNext  Amplification Buffer 300 μl 1.2 ml
AccuNext  Amplification Enzyme 36 μl 144 μl
Nuclease free water 1 ml 1 ml

 

保存与运输
保存温度:-20℃ 保存
运输温度:-20℃ 冰袋运输
示例一

采用本产品以 HL60 Cell Genomic DNA(100 pg、60 pg、15 pg)和 HL60 Cell(10 Cells 和 1 Cell)为模板进行扩增。扩增产物电泳结果如下:


扩增产物电泳结果

示例二

使用本产品扩增 100 pg 人标准品 gDNA(18号染色体缺失及21三体),并使用 AccuNext 快速 DNA 文库制备试剂盒(Illumina,100 ng 模板量)(Code No. AG12518)对扩增产物进行 DNA 文库构建,散点分布图结果显示:reads 在基因组各部位分布的比较均匀,SD 值低于 3.5,表明本产品扩增均一性好。


扩增产物全基因组拷贝数散点分布图

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